HPLC - High Performance Liquid Chromatography.
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HPLC stands for High Performance Liquid Chromatography. Its earlier name was High Pressure Liquid Chromatography because it involved use of liquid mobile phase requiring higher pressures than gases used in Gas Chromatography.
In this paper,high performance liquid chromatography with mass spectrometry (HPLC-MS) were used for the active ingredient in qualitative analysis of Apocynum leaves, based on the information of the mass spectrum molecular ion peak (M-and some characteristic fragment ions, and combined with UV spectral characteristics we initially speculated that Apocynum leaves water extract isolated to 10.
High-performance liquid chromatography, or HPLC, is a highly versatile technique that separates components of a liquid mixture based on their different interactions with a stationary phase. HPLC is an adaptation of column chromatography. In column chromatography, a column is packed with micro-scale beads called the stationary phase.
Murraya koenigii L. Spreng, a medicinally important herb of Indian origin, has been used for centuries in the Ayurvedic system of traditional Indian medicine and popularly used in Indian cuisine on a daily basis. To evaluate the quality of M. koenigii, L. leaves, a sensitive, simple and precise reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for assessment.
High-performance liquid chromatography (HPLC) was used to characterize these compounds as well as the aromatics. However, the primary aim was to get well-resolved chromatograms of each fraction. Therefore many stationary and mobile phases were tested to determine the optimum conditions for the following fractions: acids, bases, nonbasic heterocompounds; monoaromatics; diaromatics; and.